What are the requirements for RNA quality and quantity when using SMARTer Ultra Low kits?
What are the requirements for RNA quality and quantity when using SMARTer Ultra Low kits?
SMARTer Ultra low kits utilize an oligo(dT) primer for first-strand cDNA synthesis and require 10 pg–10 ng input RNA with a RIN >8 to ensure selective and efficient full-length cDNA synthesis from mRNAs.Note: DNase treatment or removal of rRNA from a total RNA prep is not required for SMARTer Ultra low kits.