• 445
• 0

How does dual indexing work?

• 759
• 0

How does In-Fusion Cloning work?

• 307
• 0

What is the efficiency of In-Fusion Cloning?

• 864
• 0

What is In-Fusion Cloning?

• 342
• 0

Is this possible and what is the advantage to growing iPS cells in suspension?

• 611
• 0

What precautions should be taken when using inosine-containing primers?

• 3037
• 0

What is touchdown PCR (TD-PCR) and when would I need to use it?

• 2623
• 0

What is the role of salt in PCR reactions?

• 6259
• 0

What is the role of magnesium in PCR, and what is the optimal concentration?

• 1116
• 0

Can DMSO be added to improve amplification of GC-rich templates?

• 2229
• 0

What is the optimal amount of DNA template that should be used for PCR?

• 828
• 0

Which extension temperature should I use, 68°C or 72°C?

• 751
• 0

What is the role of Tli RNase H in qPCR reaction?

• 497
• 0

Why is the amplicon length so limited in qPCR?

• 596
• 0

How much ROX is needed for each instrument? Why are the ROX concentrations different?

• 405
• 0

Target gene expression levels are often normalized to the expression of a “housekeeping gene” (reference gene) to correct for differences in the amount of input RNA and variations in reaction efficiency. How do I select a suitable housekeeping gene?

• 399
• 0

How can amplification of genomic DNA in total RNA samples be avoided?

• 552
• 0

Which is a better starting sample for preparing RT-qPCR calibration curves, RNA or cDNA?