How does dual indexing work?
For single-read flow cells, dual indexing introduces 16 additional cycles of sequencing: 8 cycles for the Index 1 (i7) Read and 8 cycles for the Index 2 (i5) Read.
For paired-end flow cells, dual indexing introduces 23 additional cycles of sequencing: 8 cycles for the Index 1 (i7) Read, 8 cycles for the Index 2 (i5) Read, and 7 nonimaging, chemistry-only cycles at the beginning of the i5 Read.
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