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  1. Home
  2. ProdProtocol

ProdProtocol

443 Articles
  • 82
  • 0

Will I see a batch effect if I split nuclei from a single tissue slice into multiple lanes for sequencing?

  • 80
  • 0

How is Trekker data analyzed?

  • 92
  • 0

Can I align my Trekker data to an H&E image on an adjacent section?

  • 48
  • 0

What is the recommended way to ‘bank’ samples for batch processing?

  • 81
  • 0

Can I use my own UV lamp for the Trekker workflow?

  • 96
  • 0

How deeply should I sequence Trekker libraries?

  • 74
  • 0

Will I need to optimize nuclei dissociation for my tissue?

  • 213
  • 0

What is the recommended number reads when your sample tissue does not cover the entire Tile area?

  • 333
  • 0

How much PhiX should I spike in to my library before sequencing?

  • 248
  • 0

What are the PhiX recommendations for the NovaSeq X Plus?

  • 170
  • 0

How deep should I sequence Curio Seeker libraries?

  • 198
  • 0

For the Dual Indexing Primer kit, what orientation are the sequences listed in the User Guide table?

  • 170
  • 0

Where can I find the bam file where reads are associated with bead barcodes and/or molecular barcodes?

  • 175
  • 0

What do I do if the pipeline says it cannot find my STAR reference when it clearly exists?

  • 234
  • 0

How do I fix the following error message? Remote resource not found: https://api.github.com/repos/nextflow-io/commercial_seeker/contents/main.nf

  • 161
  • 0

What do I do if the FORMATCONVERT step fails with the following error?

  • 194
  • 0

CURIOSEEKER_GEN_GENE_BARCODE_UMI_DB step failed with the following error. How do I resolve it?

  • 158
  • 0

If I have multiple regions or tissues of interest on a tile, how do I select only the beads for the region I am interested in?

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