What is the recommended way to ‘bank’ samples for batch processing?
What is the recommended way to ‘bank’ samples for batch processing?
Our protocol supports freezing the tile with the melted tissue at -80°C for up to 4 days (before UV cleavage). To process two samples in parallel, section and mount the tissue onto the first tile and start UV cleavage. If tiles with tissue sections were previously stored at -80°C, briefly melt the tile with a finger and proceed to the UV cleavage step. During the 7.5 min incubation, prepare the second tile and start UV cleavage. Perform tissue dissociation of the first tile followed by the second tile, making sure to add Buffer B within 10 min of adding Buffer A. When both samples are in Buffer B, centrifuge and complete the rest of the protocol in parallel. If desired, you may start another set of two samples while the first set undergoes the first centrifugation step.