How deeply should I sequence Trekker libraries?
How deeply should I sequence Trekker libraries?
- A: Sequencing depth for the Trekker library, like most gene expression libraries, should be adjusted according to the user guide of the relevant single-cell platform. For 10X Chromium, sequence the Trekker library at a depth of 5,000 read pairs per nucleus captured.
- For BD Rhapsody, sequence the Trekker library at a depth of 1,000 read pairs per nucleus captured.