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  2. ProdSpec

ProdSpec

280 Articles
  • 430
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What is the largest DNA fragment compatible with In-Fusion Cloning?

  • 526
  • 0

Do PCR-generated 3' A-overhangs interfere with In-Fusion Cloning?

  • 484
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What PCR polymerases are recommended for amplification of the In-Fusion cloning insert?

  • 441
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What oligonucleotide quality is required for an In-Fusion PCR primer?

  • 543
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What is the optimal length of the homologous overlap between the termini of the PCR-amplified insert and linearized cloning vector?

  • 488
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What are the requirements for a homologous overlap that will facilitate a successful In-Fusion Cloning reaction?

  • 569
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In-Fusion HD and In-Fusion Snap Assembly bundles include different PCR polymerases. Should I expect a difference in performance?

  • 532
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Does the In-Fusion Cloning method introduce errors into the sequence?

  • 601
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Is there a way to improve transduction efficiency?

  • 700
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Can cell-culture bags (e.g., X-fold bags) be coated with RetroNectin reagent?

  • 494
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Does Takara Bio provide a clinical-grade RetroNectin reagent?

  • 477
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Does RetroNectin reagent have to be filter sterilized?

  • 617
  • 0

For how long are pre-coated RetroNectin plates stable?

  • 481
  • 0

How much cytotoxicity is typically observed when using RetroNectin reagent?

  • 410
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Does RetroNectin reagent work for retroviruses packaged with different envelopes?

  • 532
  • 0

What types of viral vectors can be used with RetroNectin reagent?

  • 492
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What cell types can RetroNectin reagent be used with?

  • 530
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What is Fibronectin?

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