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  1. Home
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  3. RNA-seq general

RNA-seq general

64 Articles
  • 275
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Why is quantification of NGS libraries by qPCR better than using other methods?

  • 313
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Why should I quantify my libraries prior to sequencing?

  • 413
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How should sequencing libraries be prepared using SMARTer stranded kits?

  • 291
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What is the expected size range of fragmented, ds cDNA after library preparation with Nextera kits?

  • 288
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Do I have to scale down the Nextera XT DNA Sample Preparation Kit protocol when using 100–150 pg of ds cDNA?" "

  • 291
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Can I use more than 150 pg of ds cDNA for the Nextera XT DNA Sample Preparation Kit?

  • 277
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How do I pool cDNA libraries generated with the Low Input Library Prep kits for Illumina sequencing?

  • 338
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What is the expected size range of Covaris-sheared cDNA after library preparation?

  • 257
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What are the advantages of library prep with the ThruPLEX DNA-Seq Kit?

  • 325
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How should I set up the Peak Incident Power (W) for the Covaris S220 system?

  • 299
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What type of Covaris machine did you use to optimize the shearing parameters?

  • 317
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What is the expected size range of Covaris-sheared double-stranded (ds) cDNA?

  • 325
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What method should I use to prepare cDNA generated with SMARTer Ultra low kits for sequencing?

  • 332
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How should sequencing libraries be prepared from cDNA generated with SMARTer Ultra low kits?

  • 319
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Can I analyze unpurified double-stranded (ds) cDNA for PCR cycle optimization?

  • 326
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What can I do if I have low cDNA yield?

  • 302
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How do I determine the double-stranded (ds) cDNA yield

  • 318
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What is the expected double-stranded (ds) cDNA yield?

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