What can I do if I have low cDNA yield?
What can I do if I have low cDNA yield?
If the ds cDNA yield is less than 2 ng, you may further amplify the ds cDNA using several additional PCR cycles, based on the ds cDNA concentration (determined by Agilent 2100 Expert Software). Continue to avoid overcycling. It is preferable to use too few cycles rather than too many.An electropherogram trace of low concentration ds cDNA generated with a SMARTer Ultra low kit. The red arrow indicates the concentration as determined by Agilent 2100 Expert Software. This cDNA can be further amplified using 1–2 additional PCR cycles.