What is the expected size range of fragmented, ds cDNA after library preparation with Nextera kits?
What is the expected size range of fragmented, ds cDNA after library preparation with Nextera kits?
The Nextera kits from Illumina produce libraries with a size range of 300–1,000 bp. Please refer to the Nextera DNA Sample Preparation Guide or Nextera XT DNA Sample Preparation Guide for more specific details.Example Bioanalyzer electropherograms of cDNA libraries prepared for Illumina sequencing from 1 ng of Mouse Brain Total RNA (Panel A) or 1 ng of Human Universal Total RNA (Panel B) using a SMARTer Ultra low kit with the Nextera DNA Sample Preparation Kit and the modified Nextera protocol provided by Takara Bio. 5 ng of ds cDNA and 1 µl of Tagment DNA Enzyme (TDE1) were used for both samples. The difference in ds cDNA yield is related to the yield and size distribution occurring during initial cDNA synthesis, which may vary for different RNA sources. This will, in turn, result in different ds cDNA fragmentation patterns and yields.