Are there any special considerations that must be taken into account when performing AMPure XP purification on libraries prepared with the ThruPLEX DNA-Seq Kit?
Are there any special considerations that must be taken into account when performing AMPure XP purification on libraries prepared with the ThruPLEX DNA-Seq Kit?
Yes, we suggest using a 1:1 bead to sample ratio. Additionally, a freshly prepared solution of 80% must be used in all washing steps of the protocol. Please refer to the ThruPLEX DNA-Seq Kit User Manual for detailed instructions on AMPure XP purification of ThruPLEX DNA-Seq libraries for next-generation sequencing.