Can I use indexing primers from vendors other than Takara Bio USA?
Can I use indexing primers from vendors other than Takara Bio USA?
Indexing primers from vendors other than Takara Bio USA, are not compatible with the DNA SMART™ ChIP-Seq Kit, since within the workflow of the DNA SMART™ ChIP-Seq Kit, PCR incorporation of indexing primers requires proprietary overlaps with the first-strand cDNA template as shown in the DNA SMART™ ChIP-Seq Kit User Manual - Figure 1. Flowchart of technology in the DNA SMART ChIP-Seq Kit.