Can the DNA SMART Poly(dA) Primer anneal to internal /A -rich sequences in the genomic DNA?
Can the DNA SMART Poly(dA) Primer anneal to internal /A -rich sequences in the genomic DNA?
DNA SMART Poly(dA) Primer can anneal to the T/A -rich sequences in the genomic DNA. The level of T/A noise in the final DNA SMART™ ChIP library may vary between different target DNAs. To remove the background noise consider a software tool described by Zhao and Zheng BMC Bioinformatics (2018) 19:544 - SMARTcleaner: identify and clean off-target signals in SMART ChIP-seq analysis