• 619
• 0

What tools are available to assist in the design of PCR primers compatible with In-Fusion Cloning?

• 937
• 0

How do I design PCR primers carrying 15-nt overhangs complementary to the termini of the linearized vector or adjacent insert?

• 551
• 0

How do I generate homologous overlaps between the termini of cloning inserts and linearized vectors?

• 812
• 0

How should cells be dissociated from RetroNectin-coated plates?

• 590
• 0

After transduction of cells using RetroNectin reagent, what method do you recommend to resuspend the cells?

• 601
• 0

Is there a way to improve transduction efficiency?

• 477
• 0

Does RetroNectin reagent have to be filter sterilized?

• 617
• 0

For how long are pre-coated RetroNectin plates stable?

• 533
• 0

Can RetroNectin reagent be coated on plates that are also coated with collagen?

• 474
• 0

Can RetroNectin reagent be used in combination with Polybrene?

• 541
• 0

Why is quantification of NGS libraries by qPCR better than using other methods?

• 874
• 0

What is the expected size range of fragmented, ds cDNA after library preparation with Nextera kits?

• 564
• 0

Do I have to scale down the Nextera XT DNA Sample Preparation Kit protocol when using 100–150 pg of ds cDNA?

• 492
• 0

Can I use more than 150 pg of ds cDNA for the Nextera XT DNA Sample Preparation Kit?

• 556
• 0

Will collected cells change the volume of the cell collection buffer?

• 539
• 0

How should cells be collected for cDNA synthesis?

• 648
• 0

Can I substitute alternative products for any of the recommended additional materials?

• 1136
• 0

How can I ensure efficient cDNA purification using SPRI beads?