Will collected cells change the volume of the cell collection buffer?
Will collected cells change the volume of the cell collection buffer?
Potentially. The collected cell volume will depend on the cell diameter, so we recommend performing a preliminary cell sorting pilot study: sort 50, 100, and 1,000 cells using the appropriate nozzle and pressure settings to determine the transfer volume of the sheath fluid. If this varies from the predicted transfer volume, you may wish to adjust the volume of cell collection buffer.