Why is quantification of NGS libraries by qPCR better than using other methods?
Why is quantification of NGS libraries by qPCR better than using other methods?
By using qPCR primers that anneal to the sequencing adaptors, you can quantify just the fraction of the library capable of cluster generation. qPCR is also extremely sensitive, consuming only a small amount of your sample and making it ideal for accurate quantification of very dilute libraries.