Can I use more than 150 pg of ds cDNA for the Nextera XT DNA Sample Preparation Kit?
Can I use more than 150 pg of ds cDNA for the Nextera XT DNA Sample Preparation Kit?
In our hands, using 100–150 pg of input cDNA with the Nextera XT DNA Sample Preparation Kit generates DNA fragments with an optimal average size for Illumina cluster generation and sequencing. Using more than 150 pg of ds cDNA is not recommended since it generates significantly larger DNA fragments, which are suboptimal for Illumina cluster generation and sequencing.
For a greater cDNA input range, we recommend using the SSv4 PLUS kit (Cat. # R400752, R400753). It allows for inputs between 1-10 ng.