Can I use my own UV lamp for the Trekker workflow?

We have optimized the assay on the UV lamp provided. We cannot guarantee performance based on different UV lamps and do not recommend deviating from the lamp [...]

How deeply should I sequence Trekker libraries?

A: Sequencing depth for the Trekker library, like most gene expression libraries, should be adjusted according to the user guide of the relevant single-cell platform. For 10X Chromium, sequence the Trekker library at a depth of 5,000 read pairs per nucleus captured. For BD Rhapsody, sequence the Trekker library at a depth of 1,000 read pairs per nucleus [...]

What sequencing platforms are compatible with the Trekker kit?

We have successfully sequenced the Trekker library on the Illumina® NextSeq® 1000/2000, Illumina NovaSeq™ X, and the Element AVITI. The gene expression library can be sequenced on any sequencer supported by the single-cell platform of choice. [...]

What single-cell platforms are compatible with the Trekker kit?

This will vary depending on your scientific question. For example, if you are examining very small structures in the tissue or analyzing cell-cell interactions, you should target as many recovered nuclei as possible. However, if you are more interested in larger structural features in the tissue, a lower percentage should [...]

Will I need to optimize nuclei dissociation for my tissue?

Optimization of nuclei dissociation can help ensure the best results for your experiment. We recommend optimizing your nuclei dissociation protocol with 25 micron-thick frozen tissue sections before starting a Trekker experiment. We provide training tiles as a purchasable product for practice with off-tile tissue dissociation and Trekker tile handling. We also highly recommend consulting with the [...]