• 152
• 0

Do I need to remove ribosomal RNA prior to the SMARTer smRNA-Seq Kit workflow.

• 198
• 0

What type of Covaris machine and shearing parameters should I use?

• 141
• 0

How do I convert double-stranded SMART-Seq mRNA cDNA into the sequencing library?

• 216
• 0

What is the expected Gene Body Covergae of the SMART-Seq mRNA cDNA?

• 139
• 0

Can I use purification beads from vendor/ source other than recommended in the SMART-Seq® mRNAKit for Sequencing User Manual.

• 176
• 0

Can I use 5X First-Strand Buffer supplied with the SMARTScribe™ Reverse Transcriptase as a substitute for the 5X Ultra Low First-Strand Buffer supplied with theSMART-Seq® mRNA Kit ?

• 212
• 0

How many reactions can I perform using different size formats of the SMART-Seq® mRNA Kit ?

• 294
• 0

Why do I need to perform Positive and Negative controls?

• 232
• 0

What is the difference between SMART-Seq2 and SMART-Seq® mRNA kit?

• 181
• 0

Why are the 5’-ends of PCR Primer IIA and 3’ SMART-Seq CSD Prime IIA are blocked?

• 146
• 0

What is the advantage of using the LNA (lock nucleic acid) template-switching oligo for the SMART(er) cDNA synthesis?

• 261
• 0

What is the advantage of using SeqAmp DNA polymerase in SMART(er) cDNA synthesis for NGS?

• 290
• 0

What is the advantage of using SMARTScribe Reverse Transcriptase for the SMART-Seq mRNA cDNA synthesis?

• 132
• 0

Does the SMART-Seq mRNA cDNA synthesis require capped mRNA?

• 169
• 0

Can cells collected in QIAGEN Buffer RLT used as input for the SMART-Seq® mRNA cDNA synthesis?

• 191
• 0

Can RNA or cells stored in RNAlater be used as input for the SMART-Seq® mRNA cDNA synthesis?

• 181
• 0

Can I use a carrier or co-precipitant during purification of RNA intended for the SMART-Seq mRNA cDNA synthesis?

• 170
• 0

What can I do when input RNA exhibiting a low 260/230 OD ratio?