Is Curio Seeker compatible with Element Biosciences Aviti Sequencer?

Yes, Curio Seeker libraries are compatible with the Element Biosciences Aviti sequencer after conversion of the generated Illumina library to an Aviti library using the following protocol: Element Adept Library Compatibility Kit v1.1 https://www.elementbiosciences.com/products/adept https://www.elementbiosciences.com/products/adept [...]

What is the recommended number reads when your sample tissue does not cover the entire Tile area?

Recommended Reads and Saturation Metrics for Partial Tile Coverage For a 3x3 tile that is fully covered by the tissue section, we suggest 30 million reads for shallow sequencing or 200 million reads for deep sequencing based on data generated with mouse hippocampus. If your tissue section does not cover the entire tile area, you can lower the number of reads based on estimated % of coverage. For [...]

How much PhiX should I spike in to my library before sequencing?

NextSeq 1000/2000: 5% PhiX spike-inNextSeq 500/550: 5% PhiX spike-inNovaSeq 6000: - 5% PhiX spike-in when pooling with non-Curio Seeker libraries - 10% PhiX spike-in when pooling with only Curio Seeker [...]

What are the PhiX recommendations for the NovaSeq X Plus?

We recommend you use the same recommendations as those for the Novaseq 6000 as outlined in the user guide. src="https://customerportal.curiobioscience.com/hs-fs/hubfs/image-png-Dec-12-2023-08-53-31-8514-PM.png?width=649&height=196&name=image-png-Dec-12-2023-08-53-31-8514-PM.png [...]

How deep should I sequence Curio Seeker libraries?

Although we recommend 200-600 million paired-end reads for the 3x3 mm tile and 2-3 billion paired-end reads for the 10x10 mm tile, deeper sequencing may be required for more transcriptionally active tissue types. In the html output file from the Curio bioinformatics pipeline, the metric "median reads/UMI" indicates the approximate sequencing saturation. We recommend a value of 8-10 to reach [...]