What is difference between the Dual Indexing primer kit V1 and V2?

The primer and index sequences for these 2 kits are the same, but the concentrations are different and therefore different volumes are used. This is outlined in the protocol in section H: Tagmentation (with the Nextera XT Library Prep Kit): src="https://customerportal.curiobioscience.com/hubfs/image-png.png [...]

Do you need to optimize the permeabilization step for Curio Seeker?

There is no permeabilization step and therefore no optimization required. No fixation or permeabilization is required. This is one of the major advantages of Curio Seeker because that means you do not need to optimize the assay (permeabilization) for different tissues or species. Curio Seeker works on any species or tissue without the need for changes to the [...]

If I have low cDNA concentration, how do I re-amplify it?

Protocol for re-amplifying the cDNA if concentration is too low by Qubit quantification Create a cDNA master mix following the table below: Component 1 Reaction (μL) cDNA Amp Buffer 25 cDNA Amp Primer Mix 2 cDNA Amp Enzyme 1 Nuclease-free Water 22 uL - X uL of cDNA (e.g. 22-5 = 17 uL) Total 45 Pipet mix. Aliquot 45 uL of the master mix into a new PCR tube.Add X uL of your cDNA template into the [...]

Do I need to make any adjustments for running Human Heart tissue?

Although Seeker works well on mouse heart, the standard protocol may need to be slightly adjusted for human heart. Increase hybridization time to 30 minIncrease RT to 90 minRun 13 cycles of PCR in the second stage of cDNA [...]

What modifications do I need to make for running Curio Seeker on plant tissue?

Currently, for plant tissues, we are not aware of anything that has to be modified. The following tips may increase the chances of success. 1. During the tissue dissociation step, make sure that it is done soon after sectioning (e.g. 1-2 hours after without freezing the tile). Freezing the tile and tissue (between steps 12 and 13 of Section B on the protocol) and keeping it at -80C for a couple [...]