How do I harvest and preserve fresh frozen tissue for sectioning?

Proper handling and preservation of tissue is essential for maintaining tissue and RNA quality This document describes guidelines for harvesting and preserving fresh murine brain for -18 C cryostat sectioning and Seeker Tile analysis. It may not reflect the best conditions for your tissue type. Please use your own protocol and conditions ideal for your tissue type. Materials All materials, [...]

Is the use of the CryoCube essential? What if it is not used?

Yes, this is essential to achieve the best quality data. Generally, background noise will be reduced, thereby improving [...]

How thick should my tissue sections be for the Curio Seeker tile?

The tissue sections for the Curio Seeker Tile should be 10 microns [...]

How do we use the CryoCube?

Protocol for how to use the CryoCube Equilibrate the fresh frozen tissue and CryoCube to -18 °C in a cryostat (such as Leica CM3050S) for at least 20 minutes prior to sectioning. The optimal temperature for sectioning may vary depending on the tissue type.Prepare the hybridization reaction mix as per protocol in section B (Tissue sectioning and hybridization to the Curio Seeker tile).Mount the [...]

What kind of tissue and sample types can I use with the Curio Seeker kit?

Fresh Frozen samples are compatible with the Curio Seeker kit. The kit is also compatible with tissues from all species without any further optimization of the protocol (i.e. no permeabilization optimization). This is because the assay does not involve tissue fixation and does not require tissue permeabilization. [...]