Have you looked at repeat space since you're looking at total RNA (e.g., ERVs, etc.)?

We are in the process of looking at repeat space because we have 2 x 100 reads and are probing the total transcriptome. We don't have data to share yet, but STORM-seq could prove useful in looking at this information. SMART-Seq Stranded [...]

Have you looked at repeat space since you're looking at total RNA (e.g., ERVs, etc.)?

We are in the process of looking at repeat space because we have 2 x 100 reads and are probing the total transcriptome. We don't have data to share yet, but STORM-seq could prove useful in looking at this information.SMART-Seq Stranded [...]

When would you choose STORM-seq over 10x Genomics for single-cell sequencing technology?

We use STORM-seq and 10x Genomics as complementary approaches for answering very different kinds of questions. We use 10x Genomics when interrogating thousands to tens of thousands of cell types to understand the cell-type diversity of our populations.On the other hand, we use STORM-seq as a targeted, hypothesis-driven approach. You get thousands more genes with STORM-seq compared to 10x [...]

When would you choose STORM-seq over 10x Genomics for single-cell sequencing technology?

We use STORM-seq and 10x Genomics as complementary approaches for answering very different kinds of questions. We use 10x Genomics when interrogating thousands to tens of thousands of cell types to understand the cell-type diversity of our populations.On the other hand, we use STORM-seq as a targeted, hypothesis-driven approach. You get thousands more genes with STORM-seq compared to 10x [...]

When considering drop-out rates, does FACS lead to a significant percentage of empty wells in 384-well plates?

When looking at drop-out rates, there are a few things we need to take into consideration. First, is the potential that the sorter may not successfully place a single cell in each well. From testing at our flow cytometry core facility, we expect less than 10 empty wells in each 384-well plate. Another thing to consider is negative controls. In our 384-well plates, we include three negative [...]