How many samples will the Takara Bio SMART-Seq Stranded Kit process with these adaptations for a 384-well plate?

With the SPT Labtech mosquito HV, we have accomplished a six-fold reduction in the original volumes. Ideally, you should be able to do six-fold more reactions than the original kit.SMART-Seq Stranded [...]

How many samples will the Takara Bio SMART-Seq Stranded Kit process with these adaptations for a 384-well plate?

With the SPT Labtech mosquito HV, we have accomplished a six-fold reduction in the original volumes. Ideally, you should be able to do six-fold more reactions than the original kit.SMART-Seq Stranded [...]

How many samples will the Takara Bio SMART-Seq Stranded Kit process with these adaptations for a 384-well plate?

With the SPT Labtech mosquito HV, we have accomplished a six-fold reduction in the original volumes. Ideally, you should be able to do six-fold more reactions than the original kit.SMART-Seq Stranded [...]

How did you confirm that the SPT Labtech mosquito HV liquid handler produced accurate pipetting volumes?

It took a lot of time and effort. We performed a lot of hands-on testing with water first, then we incorporated different reagents from the kit, specifically with the varying viscosities. We had to confirm the delivery volumes were consistent and within the range we expected. Only then did we finally add samples into the protocol. SMART-Seq Stranded [...]

How did you confirm that the SPT Labtech mosquito HV liquid handler produced accurate pipetting volumes?

It took a lot of time and effort. We performed a lot of hands-on testing with water first, then we incorporated different reagents from the kit, specifically with the varying viscosities. We had to confirm the delivery volumes were consistent and within the range we expected. Only then did we finally add samples into the [...]