What rRNA does RiboGone™ - Mammalian remove?

RiboGone™ - Mammalian allows for the specific removal of rRNA sequences (5S, 5.8S, 18S, and 28S), as well as mitochondrial rRNA sequences (12S), from human, mouse, or rat total RNA.. NGS-RNA [...]

Activity-based CRISPR scanning uncovers allostery in DNA methylation maintenance machinery

Featured product. His60 Nickel Resin In this paper researchers used CRISPR scanning of the essential maintenance methylation genes DNMT1 & UHRF1 to identify mutational hotspots that yield gain-of-function [...]

A new deep-sea sponge Fibulia occiensis sp. nov. (Poecilosclerida: Dendoricellidae) from the 25° South Oceanic Core Complex in the Central Indian Ocean Ridge

Featured Product: TaKaRa Ex Premier™ DNA Polymerase Authors describe a new deep-sea sponge species, Fibulia occiensis sp. nov., from the Central Indian Ocean Ridge (CIOR) at Rodrigues Triple Junction (RTJ). The sequences of Fibulia occiensis sp. nov. were submitted to NCBI GenBank and received the accession numbers: 28S MW810076 and 18S MW810071. The 28S is 716 bp, while the 18S is 1705 bp. [...]

How does In-Fusion Cloning work?

In‑Fusion Cloning requires 15 bp of overlap at the termini of the cloning insert and linearized cloning vector, or adjacent cloning inserts if multiple inserts are to be joined simultaneously. For multiple-insert cloning, we recommend increasing the overlap to 20 bp. These 15-bp homologous overlaps can be generated by PCR amplification or oligo synthesis of either of the cloning [...]

What is the efficiency of In-Fusion Cloning?

Cloning efficiency is at least 95% for a single insert into a vector. Unlike transformation efficiency, which is merely a measure of the number of transformed colonies obtained, cloning efficiency is a measure of accuracy, providing information on the number of correct clones obtained from a cloning reaction. In-Fusion Cloning [...]