I want to purify my AAV vector. Which kit do you recommend?

We recommend the AAVpro Purification Kit (All Serotypes) as this kit can be used for any AAV serotype. View the tech notes » #6666 AAVpro purification kit (All Serotypes)AAV [...]

What are the advantages of the AAVpro Helper Free System in comparison with AAV production kits from other companies?

Our kits includes a vector that expresses hsa-miR342, a human microRNA that increases AAV production. This system increases titer by ~2-fold compared with the pRC2 vector alone. Amount of AAV particles prepared with the AAVpro Helper Free System (AAV2) is in the range of 1010 and 1011 vg/10 cm dish (vg = vector genome, as measured by real-time PCR). Please note the amount of AAV particles [...]

Do you offer AAV control vectors?

Yes, we have AAV vectors that express LacZ or ZsGreen1.AAV [...]

Infection of cultured cells cannot be confirmed; what could be the cause?

It is possible that high titers of AAV were not produced. Consider the following possible causes: The HEK 293 cell line used may affect AAV production. We recommend using the AAVpro 293T Cell Line or HEK 293T/17 (ATCC CRL 11268) cells. We have confirmed efficient AAV production for these cell lines.It is possible that transfection efficiency is low. Be sure to follow the transfection methods [...]

What cells are suitable for the production of AAV particles?

For the AAVpro Helper Free Systems, use HEK 293T or HEK 293 cells. Several HEK 293 and HEK 293T cell lines are commercially available. Viral production is highly dependent on features of the cell line. The AAVpro 293T Cell Line* or HEK 293T/17 cells (ATCC, CRL-11268) are recommended for preparation of high-titer AAV. *The AAVpro 293T Cell Line is a subclone of the transformed human embryonic [...]