What versions of packages are used in the latest Seeker Primary Analysis Pipeline?

Package Package Version License License Link nextflow >=22.04.5, <=23.04.3 Apache License 2.0 https://github.com/nextflow-io/nextflow/blob/master/LICENSE" target="_blank https://github.com/nextflow-io/nextflow/blob/master/LICENSE samtools 1.17 MIT https://github.com/samtools/samtools/blob/develop/LICENSE" target="_blank https://github.com/samtools/samtools/blob/develop/LICENSE umi_tools [...]

What is the structure of read 1 and read 2 in the Seeker data?

As shown in the figure below, both molecular and cellular barcodes are in Read 1. mRNA information is in Read 2. [...]

What is the unit of the spatial coordinates from the Seeker Primary Analysis Pipeline? How do I convert them to µm?

The coordinate s generated by the Seeker Primary Analysis P ipeline are in pixel s ( 1 pixel = ~0.647 µ m ) [...]

What's the pore size of Capturem membrane?

The pores on the Capturem membrane is 1.2um before applying chemistry. Pore size after chemistry is not [...]

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