What is included in the Guide-it SNP Screening Kit?
What is included in the Guide-it SNP Screening Kit?
The Guide-it SNP Screening Kit includes all necessary reagents for extraction and amplification of genomic DNA from mammalian cell samples, and for performing the Guide-it Flapase enzymatic assay:
MightyPrep Reagent for DNA (Store at 4°C)
MightyPrep Reagent for DNA
Guide-it SNP Control Set (Store at –20°C)
Guide-it SNP Positive Control Mix
Guide-it SNP Negative Control Mix
Guide-it Flap Reagents (Store at –20°C)
Terra PCR Direct Polymerase Mix
2X Terra PCR Direct Buffer (with Mg2+, dNTP)
Dilution Buffer
RNase-free Water
Annealing Buffer
Flapase Buffer
Guide-it Flapase
Guide-it Flap Detector (40X)
In addition to user-provided oligos specific for each assay, performing the assay requires a thermal cycler and fluorescence plate reader.