Can the Guide-it SNP Screening Kit only be used to detect single-nucleotide substitutions? Would it be suitable for detecting longer insertions?
Can the Guide-it SNP Screening Kit only be used to detect single-nucleotide substitutions? Would it be suitable for detecting longer insertions?
The current version of the Guide-it SNP Screening Kit can only be used to detect single-nucleotide substitutions, not longer insertions.
We are, however, in the process of developing a new version of the kit that will enable detection of longer insertions using two Guide-it Flap Detectors in tandem. One detector yields a red fluorescent signal while the other one generates a green signal, allowing simultaneous detection of seamless insertions at both the 5' and 3' ends of the recombinant sequence. We have applied this updated approach for detecting the fusion of a myc tag to an endogenous gene in hiPSCs (a poster including this data is available for download). http://www.takarabio.com/Documents/Posters%20-%20R%20and%20D/Gene%20Function/2018-CSHL-pos...