What cell densities are suitable for analysis with the Guide-it SNP Screening Kit?
What cell densities are suitable for analysis with the Guide-it SNP Screening Kit?
Clonal cell lines in a 96-well plate can have different growth rates, and therefore the protocol has been optimized for a wide range of cell densities (from 2 x 104 to 2 x 105 cells per well).
However, if this assay is being performed for the detection of homologous recombination we recommend that the assay be performed with genomic DNA that has been purified using NucleoSpin Tissue Columns (Cat. # 740952.250S).