Can the protocol for the Guide-it SNP Screening Kit be adapted for use with 384-well plates?
Can the protocol for the Guide-it SNP Screening Kit be adapted for use with 384-well plates?
We have not tested the kit protocol using 384-well plates; however, theorizing such an adaptation, the only modification we can foresee at this time is a reduction in reaction volumes. Please note that some troubleshooting may be required to ensure successful setup of the PCR reaction and enzymatic assay and to confirm that the resulting fluorescent signal is strong enough to be detected using your chosen method. If you need more assistance with this, please contact our Technical Support team.