Can I use the kit if I have no idea what the substitution is?
Can I use the kit if I have no idea what the substitution is?
Each assay performed with the Guide-it SNP Screening Kit is designed to detect a specific single-nucleotide substitution at a particular genomic location. The sequence of the flap-probe oligo determines which nucleotide will be detected at a chosen target site. To be able to identify any nucleotide at a given site, one must perform four independent assays, with each assay using a different flap-probe oligo designed to specifically detect a G, C, A, or T at the target site (i.e., one specific flap-probe oligo per possible nucleotide).