How do you explain the presence of introns in the final SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian cDNA library?
How do you explain the presence of introns in the final SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian cDNA library?
The SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian uses a random priming strategy that generates cDNA from coding, noncoding, and alternatively spliced RNAs that may carry introns. Additionally, previously unannotated transcripts may be assigned as introns by your software's default.