What type of Covaris machine and shearing parameters should I use?
What type of Covaris machine and shearing parameters should I use?
Covaris shearing parameters for the SMART-Seq cDNA , were optimized using a Covaris S220 Focused-ultrasonicator to obtain size distribution of 100–500 bp with a peak of approximately 200 b. Use 130-?l Covaris tube, containing 75 ?l SMART-Seq cDNA in the Elution Buffer, set-up the following parameters: Peak Power – 175, Duty-10%, Burst Cycle – 200, Time(min)-5 min, Mode-Frequency Sweeping