Can RNA or cells stored in RNAlater be used as input for the SMART-Seq® mRNA cDNA synthesis?
Can RNA or cells stored in RNAlater be used as input for the SMART-Seq® mRNA cDNA synthesis?
RNAlater is NOT compatible with the SMART-Seq® mRNA cDNA synthesis, since it denatures proteins. RNA from cells stored in RNAlater must be purified prior to use as input for the SMART-Seq® Ultra® Low cDNA synthesis.