How do I convert double-stranded SMART-Seq mRNA cDNA into the sequencing library?
How do I convert double-stranded SMART-Seq mRNA cDNA into the sequencing library?
SMART-Seq mRNA cDNA can be converetd into the Illumina sequencing library using Takara Bio USA SMART Seq Library Prep Kit, Covaris shearing followed by library construction using the ThruPLEX DNA-Seq Kit, Illumina Nextera® XT DNA Sample Preparation Kit (using as input 100-300pg SMART Seq cDNA and following the standard Nextera XT protocol)