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  2. AssayPrinciple

AssayPrinciple

118 Articles
  • 345
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Why are homologous overlaps important for In-Fusion Cloning reactions?

  • 387
  • 0

What is the optimal length of the homologous overlap between the termini of the PCR-amplified insert and linearized cloning vector?

  • 482
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How do I design PCR primers carrying 15-nt overhangs complementary to the termini of the linearized vector or adjacent insert?

  • 416
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How do I generate homologous overlaps between the termini of cloning inserts and linearized vectors?

  • 347
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What are the requirements for a homologous overlap that will facilitate a successful In-Fusion Cloning reaction?

  • 371
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What are Very Late Antigen-4 (VLA-4) and Very Late Antigen-5 (VLA-5)?

  • 366
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How does RetroNectin reagent colocalize viral particles and cells? Where do the virus particles bind?

  • 330
  • 0

What are self-inactivating (SIN) retroviral vectors (Retro-X Q Vectors)?

  • 452
  • 0

What are general considerations for cDNA synthesis directly from cells?

  • 348
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Why do I need to remove ribosomal RNA?

  • 269
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Why are the 5’-ends of PCR Primer IIA and 3’ SMART-Seq CSD Prime IIA are blocked?

  • 285
  • 0

What is the mechsmism of the SMART-Seq mRNA cDNA synthesis?

  • 172
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What rRNA species are not removed by the ZapR

  • 320
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What is the mechanism of the removal of rRNA sequences within the workflow of the SMART-Seq® Stranded Kit?

  • 359
  • 0

What enzyme is used to passage cells cultured in DEF-CS?

  • 314
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What are the viral delivery systems of Takara Tet system technology

  • 293
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What's inducible coexpression and what are the advantages?

  • 332
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What are the features of the Tet-On 3G transcriptional activator: highest sensitivity to doxycycline

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