How does RetroNectin reagent colocalize viral particles and cells? Where do the virus particles bind?
How does RetroNectin reagent colocalize viral particles and cells? Where do the virus particles bind?
RetroNectin reagent can enhance retroviral-mediated gene transfer into mammalian cells by colocalizing retrovirus particles and target cells. Colocalization is thought to be accomplished by direct binding of viral particles to sequences in the heparin-binding domain, and interaction of cellular integrins VLA-4 and/or VLA-5 with the RetroNectin molecule.