What is the mechsmism of the SMART-Seq mRNA cDNA synthesis?
SMART-Seq mRNA kit utilizing oligo(dT) priming for the first-strand cDNA synthesis generates full-length double-stranded cDNA originated from full-length polyA RNAs. SMART Template Switching Oligo ensures an efficient cap-independent incoporporation of the 5’-end of mRNA contributing to uniform gene body coverage.
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