What's inducible coexpression and what are the advantages?
What's inducible coexpression and what are the advantages?
We offer two types of Tet-inducible gene coexpression. Both types give you a choice of expressing a target gene and a reporter gene, or two target genes.
Bidirectional Tet-On 3G systems
-Bidirectional vectors contain a specialized TRE3G promoter (PTRE3G-BI) that allows for inducible, simultaneous, and equivalent expression of two transgenes. Bidirectional vectors allow you to monitor expression of a target gene indirectly, via co-induced expression of a bright green or red fluorescent protein. Both proteins are expressed equivalently from the bidirectional Tet promoter.
-Bicistronic Tet-On 3G systems
Bicistronic (IRES-containing) vectors allow you to express two proteins simultaneously but separately, from the same RNA transcript. Bicistronic vectors are ideal for stable clone selection, because you can monitor expression of your transgene together with a bright fluorescent protein (mCherry or ZsGreen1). Please note that expression of the proteins that are upstream and downstream of the IRES sequence varies
