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  2. GeneralConcept

GeneralConcept

61 Articles
  • 273
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What are the effects of chromatin configuration on HDR efficiency?

  • 227
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Does the HDR insertion site need to be directly next to the PAM?

  • 435
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How do ssODNs differ from ssDNA? When is it better to use one vs. the other?

  • 236
  • 0

What is HDR for Gene Editing?

  • 231
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What is metal ion transfer?

  • 234
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What should be done if the protein contains a tag in the middle of the sequence, i.e., not a terminal tag?

  • 252
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How can the yield of purified HAT- or 6xHis-tagged fusion protein be increased?

  • 241
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Does increasing the number of histidine residues beyond the usual six affect the ability of a protein to bind to TALON resin?

  • 282
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Can I perform single-read runs and still obtain both index sequences?

  • 340
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Can I do paired-end sequencing with single-indexed libraries?

  • 516
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What is the difference between single and paired-end reads?

  • 287
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How can I mitigate index crosstalk or ""sample bleeding"" issues?

  • 494
  • 0

What is index hopping?

  • 390
  • 0

What are the advantages of using dual- versus single-indexed libraries?

  • 296
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How does dual indexing work?

  • 262
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How does reprogramming method affect the application of DEF-CS system?

  • 243
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What are the candidates for substitute pluripotency tests?

  • 221
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What are the common pluripotency markers?

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