Can I perform single-read runs and still obtain both index sequences?
Can I perform single-read runs and still obtain both index sequences?
Yes, both index sequences can be obtained from single-read runs. Please select the appropriate workflow based on the type of flow cell (e.g., single-read or paired-end) so that the correct chemistry is used. If you are using a single-read flow cell, the TruSeq® Dual Index Sequencing Primer Box, Single-Read (Illumina, Cat. # FC-121-1003) is necessary to sequence both index reads.