Does increasing the number of histidine residues beyond the usual six affect the ability of a protein to bind to TALON resin?
Does increasing the number of histidine residues beyond the usual six affect the ability of a protein to bind to TALON resin?
Based on Takara Bio's research with TALON resin, increasing the number of histidine residues beyond the usual six does not increase the extent of binding or yield of the tagged protein. We have also found that the use of multiple types of tags does not improve purification results.