What are the advantages of using dual- versus single-indexed libraries?
What are the advantages of using dual- versus single-indexed libraries?
There are several advantages of using dual indexes:
Dual indexes increase the accuracy of sample identification by adding another identifier to minimize issues caused by index-hopping events
Dual-indexed libraries allow higher multiplexing and the ability to run more samples per lane
Dual-indexed libraries are sequenced with 2 additional reads, termed Index 1 (i7) Read and Index 2 (i5) read