Can I do paired-end sequencing with single-indexed libraries?
Can I do paired-end sequencing with single-indexed libraries?
Yes, paired-end sequencing can be done with single-indexed libraries. This is because single vs. dual-indexed adapters have nothing to do with sequencing due to how the sequencing is performed. Sequencing is done using Read Primer 1 or Read Primer 2, both of which are present in all libraries. You can also do single reads even if you use dual-indexed kits.