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  1. Home
  2. AssayTroubleshooting

AssayTroubleshooting

53 Articles
  • 572
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Can I modify the length of the homologous overlap? Will a longer overlap improve In-Fusion Cloning efficiency?

  • 601
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For In-Fusion Cloning, is it a problem if the 15-bp region of homology is present more than once in the vector? Will multiple recombination products result?

  • 543
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What are the most common artifacts of cDNA synthesis with SMARTer kits?

  • 874
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What is the expected size range of fragmented, ds cDNA after library preparation with Nextera kits?

  • 630
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How do you explain the presence of introns in the final SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian cDNA library?

  • 547
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What are abnormal Bioanalyzer traces for SMARTer stranded kits?

  • 600
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What are abnormal Bioanalyzer traces for SMARTer Ultra low kits?

  • 976
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What are abnormal Bioanalyzer traces for all SMARTer kits?

  • 590
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What can I do if I have low cDNA yield?

  • 303
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What is the origin of prominent peaks in the electropherogram of the SMARTer smRNA library generated from total RNA?

  • 371
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Will there be genomic DNA contamination in the SMART-Seq Stranded libraries, generated from whole cells?

  • 596
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I observe dome-like structures in Cellartis enhanced hiPS-HEP cell culture. Is this normal?

  • 528
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I observe droplets in Cellartis enhanced hiPS-HEP cell culture. Is this normal?

  • 406
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What is the optimal cargo-to-reagent ratio and amount for transfection?

  • 533
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Infection of cultured cells cannot be confirmed; what could be the cause?

  • 473
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Upon addition of AAV Extraction Solution B, the solution turned pink; is this a problem?

  • 470
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Infection of cultured cells cannot be confirmed; what could be the cause?

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