Infection of cultured cells cannot be confirmed; what could be the cause?
Infection of cultured cells cannot be confirmed; what could be the cause?
It is possible that high titers of AAV were not produced. Consider the following possible causes:
The HEK 293 cell line used may affect AAV production. We recommend using the AAVpro 293T Cell Line or HEK 293T/17 (ATCC CRL 11268) cells. We have confirmed efficient AAV production for these cell lines.
It is possible that transfection efficiency is low. Be sure to follow the transfection methods outlined in the user manual