Can I modify the length of the homologous overlap? Will a longer overlap improve In-Fusion Cloning efficiency?
Can I modify the length of the homologous overlap? Will a longer overlap improve In-Fusion Cloning efficiency?
Current In-Fusion Cloning reaction conditions favor a 15-bp homologous overlap for single-insert cloning, and a 20-bp homologous overlap for multiple-insert cloning. We do not recommend using overlaps shorter than 12 bp or longer than 21 bp.