Infection of cultured cells cannot be confirmed; what could be the cause?
Infection of cultured cells cannot be confirmed; what could be the cause?
It is possible that high titers of AAV were not produced. Consider the following possible causes:
- The HEK 293 cell line used may affect AAV production. We recommend using the AAVpro 293T Cell Line or HEK 293T/17 (ATCC CRL 11268) cells. We have confirmed efficient AAV production for these cell lines.
- It is possible that transfection efficiency is low. Be sure to follow the transfection methods outlined in the user manual.