Will the Trekker kit work with FFPE (formalin-fixed paraffin-embedded) tissue?

The Trekker kit is currently compatible with fresh frozen tissues. Please contact your local Takara Bio representative for more information regarding compatibility with FFPE [...]

Will the Trekker protocol work on my tissue?

The Trekker kit has successfully been applied to samples from mouse brain, kidney, liver, embryo, colon, lung, testis, spleen and human brain, breast cancer, and melanoma. Please contact your local Takara Bio representative for more information on compatible tissue types. The Trekker protocol should be compatible with most tissues, but some may require optimization of nuclei dissociation to [...]

How many nuclei should I expect from my tissue section after tissue dissociation?

Previous ranges observed post-dissociation: #_msocom_1 [CC1] Mouse brain: ~120,000 nuclei Mouse spleen: ~0.5–1 x 106 nuclei Mouse embryo (E11): ~250,000 nuclei #_msoanchor_1 [CC1]once we have the tissue table we can point them to that. for now this is fine. [...]

What range of nuclei recovery rate should I expect with the Trekker kit?

End-to-end nuclei recovery, from tissue dissociation to positioning, depends mainly on three factors: 1. Recovery rate from nuclei dissociation—typically 50-70% 2. Recovery rate from the single-cell sequencing platform—typically 50-70% for the most efficient platforms, but varies by platform 3. Positioning rate by Trekker spatial barcodes—typically 60-90% nuclei positioned with at least one [...]

What are the dimensions of 740488?

length: 230 mm; depth: 158mm; height: 100 mm src="https://cdn.livehelpnow.net/clients/36168/kb/image_720974dc-c556-4a2e-a415-528858efdada.png" id="6f78f7a3-a651-4cd4-ad9a-8cbfa7ec42c5" alt="image" width="90%" height="auto" data-image="6f78f7a3-a651-4cd4-ad9a-8cbfa7ec42c5 [...]